VhuD Facilitates Electron Flow from H 2 or Formate to Heterodisulfide Reductase in Methanococcus maripaludis
نویسندگان
چکیده
منابع مشابه
Protein complexing in a methanogen suggests electron bifurcation and electron delivery from formate to heterodisulfide reductase.
In methanogenic Archaea, the final step of methanogenesis generates methane and a heterodisulfide of coenzyme M and coenzyme B (CoM-S-S-CoB). Reduction of this heterodisulfide by heterodisulfide reductase to regenerate HS-CoM and HS-CoB is an exergonic process. Thauer et al. [Thauer, et al. 2008 Nat Rev Microbiol 6:579-591] recently suggested that in hydrogenotrophic methanogens the energy of h...
متن کاملProspects for Hydrogen Production from Formate by Methanococcus maripaludis
Most methanogenic Archaea are hydrogenotrophic, specializing in the use of hydrogen to reduce CO2 to methane. Even though these organisms have multiple hydrogenases that use hydrogen, there is good potential to use them for the net production of hydrogen. This is because some species can use alternative electron donors, most notably formate, and because some species have nitrogenases that can p...
متن کاملHydrogenases of Methanococcus maripaludis
The methanogens catalyze a major component of the Earth’s H2 cycle. They are especially active in anaerobic environments where they are the primary consumers of fermentatively produced H2. These strictly anaerobic Archaea have evolved unique adaptations to H2 metabolism, many of which are poorly understood. Our research examines the enzymes and pathways in H2 metabolism in methanogens, especial...
متن کاملFunction and regulation of the formate dehydrogenase genes of the methanogenic archaeon Methanococcus maripaludis.
Methanococcus maripaludis is a mesophilic species of Archaea capable of producing methane from two substrates: hydrogen plus carbon dioxide and formate. To study the latter, we identified the formate dehydrogenase genes of M. maripaludis and found that the genome contains two gene clusters important for formate utilization. Phylogenetic analysis suggested that the two formate dehydrogenase gene...
متن کاملReconstitution of a formate-NADP+ oxidoreductase from formate dehydrogenase and a 5-deazaflavin-linked NADP+ reductase isolated from Methanococcus vannielii.
The formate-dependent reduction of NADP+ by extracts of Methanococcus vannielii is catalyzed by a coupled system consisting of formate dehydrogenase, a 5-deazaflavin cofactor, and 5-deazaflavin-dependent NADP+ reductase. All three components were purified from crude extracts of M. vannielii. Recombination of these components reconstituted the formate-NADP+ oxidoreductase system. The formate deh...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2013
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00895-13